The effect of high-calorie meal consumption on oxidative stress and endothelial dysfunction in healthy male adults Short title: Postprandial response of triglycerides

2 SUMMARY Several authors have reported the association of postprandial hypertriglyceridemia with oxidative stress, systemic inflammation and endothelial dysfunction. Our aim was to investigate the effect of high-calorie meal on blood markers of oxidative stress and endothelial dysfunction and the association of APOA5-1131T/C and-250G/A hepatic lipase (HL) polymorphisms with postprandial triglyceride response. This study included 102 healthy male volunteers. All participants consumed a high-calorie meal (800 calories, 50 g fat, 28 g protein, 60 g carbohydrates). and ICAM-1 were measured at fasting state and postprandially. APOA5-1131T/C and-250G/A HL polymorphisms were also determined. Concentration of triglycerides, HDL-cholesterol, LDL-cholesterol, TAS and ICAM-1 differed significantly between the fasting state and postprandial measurements (P<0.001). However, those differences were within the limits of analytical imprecision. Other parameters did not change 3 hours after the meal. Triglycerides response did not differ respective to the APOA5 and HL polymorphisms. Family history of hypertension and acute myocardial infarction were associated with higher postprandial triglyceride concentrations. 3 Postprandial hypertriglyceridemia is not associated with increased concentrations of hsCRP, TAS and ICAM-1. Furthermore, APOA5-1131T/C and-250G/A HL polymorphisms are not associated with different postprandial triglyceride response. 4 List of Abbreviations: APOA5, apolipoprotein A5 BMI, body mass index CAD, coronary artery disease ELISA, enzymimmunoassay HL, hepatic lipase hsCRP, high-sensitivity C-reactive protein ICAM-1, intercellular adhesion molecule-1 PAI-1, plasminogen activator inhibitor type 1 PCR, polymerase chain reaction RFLP, restriction fragment length polymorphism RT-PCR, real time PCR TAS, total antioxidant status TNF-α, tumor necrosis factor-α VCAM-1, vascular cell adhesion molecule-1